Endophytic fungi in roots of native orchids of rupestrian grasslands (campos rupestres) in Serra do Cipó, Brazil

Autores

  • Letícia Miranda Universidade Federal de Viçosa
  • Marlon Correa Pereira Universidade Federal de Viçosa https://orcid.org/0000-0001-5961-0610
  • Tomás Gomes Reis Veloso Universidade Federal de Viçosa
  • Natália Tartarine Universidade Federal de Viçosa
  • Samira Barcelos Gontijo de Carvalho Universidade Federal de Viçosa
  • Maria Catarina Megumi Kasuya Universidade Federal de Viçosa

DOI:

https://doi.org/10.21826/2446-82312021v76e2021021

Palavras-chave:

Endófitos, Fungo micorrízico, Orquídea endêmica

Resumo

Fungos endofíticos radiculares são essenciais para o ciclo de vida das orquídeas. Muitos deles atuam como promotores de crescimento da planta hospedeira e os endófitos micorrízicos são indispensáveis a germinação de sementes. Este estudo objetivou avaliar a riqueza de fungos endofíticos cultiváveis de cinco orquídeas nativas de Campos Rupestres na Serra do Cipó, Brasil. Raízes saudáveis foram amostradas e os endófitos isolados. Cada isolado teve seu DNA total extraído e a região ITS (Internal Transcribed Spacer) amplificada e sequenciada. A análise de 32 sequências fúngicas permitiram a identificação de 25 espécies pertencentes aos filos Ascomycota e Basidiomycota. Nove isolados pertenciam aos gêneros Sebacina Tul. & C. Tul. and Tulasnella J. Schröt. do filo Basidiomycota, que são importantes gêneros de fungos micorrízicos de orquídeas. A mesma espécie fúngica foi obtida de diferentes orquídeas crescendo em substratos distintos. A identificação molecular dos fungos indicou alta riqueza de endófitos sustentando o ciclo de vida das orquídeas em campos rupestres. 

 

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Biografia do Autor

Letícia Miranda, Universidade Federal de Viçosa

Biological Science and Health Institute, Rio Paranaíba Campus, Federal University of Viçosa. Department of Microbiology, Federal University of Viçosa.

Marlon Correa Pereira, Universidade Federal de Viçosa

Biological Science and Health Institute, Federal University of Viçosa, Rio Paranaíba Campus.

Tomás Gomes Reis Veloso, Universidade Federal de Viçosa

Department of Microbiology, Federal University of Viçosa.

Natália Tartarine, Universidade Federal de Viçosa

Biological Science and Health Institute, Rio Paranaíba Campus, Federal University of Viçosa.

Samira Barcelos Gontijo de Carvalho, Universidade Federal de Viçosa

Biological Science and Health Institute, Rio Paranaíba Campus, Federal University of Viçosa.

Maria Catarina Megumi Kasuya, Universidade Federal de Viçosa

Department of Microbiology, Federal University of Viçosa.

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Publicado

2021-10-05

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Miranda, L., Pereira, M. C., Veloso, T. G. R., Tartarine, N., Carvalho, S. B. G. de, & Kasuya, M. C. M. (2021). Endophytic fungi in roots of native orchids of rupestrian grasslands (campos rupestres) in Serra do Cipó, Brazil. Iheringia, Série Botânica., 76. https://doi.org/10.21826/2446-82312021v76e2021021

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