Tissue culture optimization for Lallementia royleana L. an important medicinal plant

Autores

  • Tariq Mahmood Quaid-i-Azam University. Department of Plant Sciences
  • Huma Arshad Quaid-i-Azam University. Department of Plant Sciences
  • Wasim Akhtar University of Azad Jammu and Kashmir Muzaffarabad. Department of Botany
  • Muhammad Ilyas University of Swabi KP. Department of Botany
  • Tariq Habib University of Azad Jammu and Kashmir Muzaffarabad. Department of Botany
  • Zabta Khan Shinwari Quaid-i-Azam University. Department of Plant Sciences

DOI:

https://doi.org/10.21826/2446-82312021v76e2021009

Palavras-chave:

cultura in vitro, micropropagação, tiro

Resumo

O estudo foi realizado para desenvolver um protocolo eficiente para a cultura in vitro de Lallementia royleana e para analisar o efeito de vários hormônios em sua capacidade de cultura. Na organogênese indireta, sua capacidade calogenética foi avaliada com os melhores resultados (100%) com 1 mg L-1 de benzil aminopurina (BAP) e 2 mg L-1 e combinação de ácido indolacético (IAA). Na regeneração indireta, explantes de pecíolo de folhas, nós e entrenós foram cultivados para filmagem em várias concentrações de BAP e cinetina (KIN). O valor máximo (80%) foi obtido das partes nodais com 1,0 mg L-1 de BAP e 0,5 mg L-1 de KIN. Da mesma forma, o melhor enraizamento in vitro (24 raízes) foi obtido com 1 mg L-1 de ácido naftaleno acético (NAA). L. royleana apresentou maior regeneração por regeneração direta do que por calogênese. Observou-se variabilidade significativa (p = 0,002625) entre indução de calos, partes de plantas e combinações hormonais. Os explantes de nódulos e internódios apresentaram respostas observáveis de calos em combinações de ácido 2, 4-diclorofenoxiacético (2,4-D) com NAA e BAP com IAA.

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Biografia do Autor

Tariq Mahmood, Quaid-i-Azam University. Department of Plant Sciences

Part of M. Phil Dissertation of the second author Department of Plant Sciences, Quaid-i-Azam University,  Islamabad, Pakistan.

Huma Arshad, Quaid-i-Azam University. Department of Plant Sciences

Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan

Wasim Akhtar, University of Azad Jammu and Kashmir Muzaffarabad. Department of Botany

Department of Botany, University of Azad Jammu and Kashmir Muzaffarabad, Pakistan.

Muhammad Ilyas, University of Swabi KP. Department of Botany

Department of Botany, University of Swabi KP, Pakistan

Tariq Habib, University of Azad Jammu and Kashmir Muzaffarabad. Department of Botany

Department of Botany, University of Azad Jammu and Kashmir Muzaffarabad, Pakistan.

Zabta Khan Shinwari, Quaid-i-Azam University. Department of Plant Sciences

Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan

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Publicado

2021-05-25

Como Citar

Mahmood, T., Arshad, H., Akhtar, W., Ilyas, M., Habib, T., & Shinwari, Z. K. (2021). Tissue culture optimization for Lallementia royleana L. an important medicinal plant. Iheringia, Série Botânica., 76. https://doi.org/10.21826/2446-82312021v76e2021009

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