In vitro propagation of coral-da-serra (Siphocampylus betulifolius) from nodal segments
Keywords:
Campanulaceae, micropropagation, cloning, organogenesisAbstract
Siphocampylus betulifolius (Cham.) G. Don has the potential for indoor decoration, especially for presenting continuous flowering in partially shaded places. A sequence of experiments was carried out in order to identify the conditions to its in vitro propagation. Micropropagation was executed in MS medium with 70% of the composition of salts and vitamins, and with 30 g.L-1 of sucrose, 7.5 g.L-1 of agar and pH adjusted to 5.8 with different concentrations of naphthaleneacetic acid (ANA) and benzyladenine (BAP) in each step. The induction of nodal segments in 0.1 mg ANA and 0.5 to 1.5 mg.L-1 of BAP was satisfactory. Multiplication was made with 0.5 mg.L-1 of BAP. Rooting was significantly higher with 1 mg.L-1 of ANA and 48% of the plants survived to acclimatization under intermittent nebulization. We concluded that the propagation of Siphocampylus betulifolius can be accomplished through nodal segments cultured in vitro.
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