Cryoprotector on cryopreservation of <i>Passiflora mucronata</i> Lam
DOI:
https://doi.org/10.21826/2446-82312019v74e2019008Keywords:
dimethylsulfoxide, ornamental, passizon fruit, sucrose.Abstract
Brazil is one of the centers of diversity of the genus Passiflora L. and therefore has great potential for the utilization of the genetic resources in agronomy. Passiflora mucronata Lam. is a wild species that presents great characteristics for ornamental use. This work aimed to evaluate the effect of different concentrations of cryoprotectants dimethylsulfoxide (DMSO) and sucrose (0, 7, 14, 21 and 28%) on the cryopreservation of Passiflora mucronata seeds. After the application of the cryoprotectants, the seeds were stored in liquid nitrogen for 5 days, thawed, and later, seeded in vermiculite and maintained at a temperature of 27.7 ± 1.9°C. The cryoprotectant type and the application of increasing doses affect the physiological quality of seeds after storage in liquid nitrogen. Cryopreservation may be used for the long-term conservation of seeds, without the use of cryoprotectants.Downloads
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